Application of Concentrated Growth Factors Membrane for Human Umbilical Cord Wharton’s Jelly Mesenchymal Stem Cell Differentiation towards Keratinocytes

Concentrated growth factors are extracted from platelet-rich plasma obtained healthy adult veins by physical gradient centrifugation, and the activated platelets release various cytokines, which can be further converted into concentrated liquid or gel preparations different centrifuge tubes. These widely used in clinical treatments fields, such as dentistry, dermatology surgery. In this article, platelet-poor six adults were pressed a membrane membrane. We examined whether 3D fibrin mesh within could bioscaffold for human Wharton’s jelly umbilical cord stem cell line HaCaT to attach, proliferate form epidermal-like tissue. also aimed implant cells on membrane, compare characteristics of similar tissues after 4 weeks vitro culture. The results showed that mesenchymal cells, implanted upper surface subsequent attachment proliferation. After ex vivo tissue culture, multi-layer formed containing factors. This had minimum thickness 89.91 µm maximum 204.19 µm, mean ± SD = 144.36 43.14 µm. Sections these immunohistochemical staining. found 79.8% 7.2% expressed pancytokeratin marker, 29.5% 9.4% P63 71.7% 3.9% vimentin marker. same attach tissue, 63.17 100.26 74.05 13.44 88.1% 4.9% 63.6% 11.4% 79% 9.9% Also, it distribute whole-tissue sections. 9.7% 2.4% 7.45% 1.9% maker, 95.9% 3.7% terms percentage expressing pancytokeratin, P63, markers, there was significant difference between cultivating scaffold scaffolds. (cultivating membrane) no difference. suggested use (composed mesh, cytokines) an effective self-contained differentiate towards keratinocytes-like cells. future, donors’ own applied auxiliary tool autologous regeneration.